100bp DNA Ladder H3 - 500 µl

Overview

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.

Source

PCR products and double-stranded DNA digested with appropriate restriction enzymes are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 10mM EDTA.

Specifications

Size

50 μg / 500 μl

Range

100-1,500 base pairs

Number of Bands

11

Concentration

100 μg/ml

Storage

• Store at 25°C for 6 months
• Store at 4°C for 12 months
• Store at -20°C for 24 months

Tracking dyes

orange G & xylene cyanol FF

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