1kb DNA Ladder - 500 µl

Overview

A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 13 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 250-10,000 base pairs. The 1K and 3K bands have increased the intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.

Source

PCR products and double-stranded DNA digested with appropriate restriction enzymes are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Specifications

Size

50 μg / 500 μl

Range

250-10,000 base pairs

Number of Bands

13

Concentration

100 μg/ml

Recommended Load

5 μl/well

Tracking dyes

bromophenol blue & xylene cyanol FF

Storage

• Store at 25°C for 6 months
• Store at 4°C for 12 months
• Store at -20°C for 24 months

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