
DNA/RNA Isolation Kits
Blood Genomic DNA Extraction Mini Kit
Catalogue no.
Principlemini spin column (silica matrix)
Sample size
- Up to 200 μl whole blood, serum, plasma, body fluids.
- Up to 5 x 10⁶ cultured cells.
Operation time30 minutes
Binding capacityup to 60 μg total DNA / column
Toll free +91-1800-890-4669
Offices in Delhi, Faridabad & Lucknow
Strict quality practices
Specifications
- Principle
- mini spin column (silica matrix)
- Sample size
- Up to 200 μl whole blood, serum, plasma, body fluids.
- Up to 5 x 10⁶ cultured cells.
- Operation time
- 30 minutes
- Binding capacity
- up to 60 μg total DNA / column
- Expected yield
- 4 ~ 8 μg / prep
- Column applicability
- centrifugation and vacuum
- Minimum elution volume
- 50 μl
Kit components
| HBBGK050 | HBBGK100 | HBBGK200 | HBBGK300 | |
|---|---|---|---|---|
| BG Buffer | 15 ml | 30 ml | 60 ml | |
| GW Buffer (concentrate) | 22 ml | 44 ml | 88 ml | |
| Wash Buffer (concentrate) | 10 ml | 20 ml | 40 ml | |
| Elution Buffer | 15 ml | 30 pcs | 60 ml | |
| Proteinase K | 11 mg | 11 mg x 2 | 11 mg x 4 | |
| BG Mini Column | 50 pcs | 100 pcs | 200 pcs | |
| Collection Tube | 100 pcs | 200 pcs | 400 pcs | |
| Elution Tube | 50 pcs | 100 pcs | 200 pcs | |
| User Manual | 1 | 1 | 1 |
Handling notes
- Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
- Add 1.1 ml sterile ddH2O to Proteinase K tube to make a 10 mg/ml stock solution. Make sure that Proteinase K has been completely dissolved. Store the stock solution at 4 ℃ .
- Add ethanol (96- 100 %) to GW Buffer and Wash Buffer when first open.
- Prepare dry baths or water baths before the operation. One to 60℃ for step 2. Lysis.
Protocol
Downloads
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