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DNA/RNA Isolation Kits

Blood Genomic DNA Extraction Mini Kit

Catalogue no.
Principlemini spin column (silica matrix)
Sample size
  • Up to 200 μl whole blood, serum, plasma, body fluids.
  • Up to 5 x 10⁶ cultured cells.
Operation time30 minutes
Binding capacityup to 60 μg total DNA / column
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Strict quality practices

Specifications

Principle
mini spin column (silica matrix)
Sample size
  • Up to 200 μl whole blood, serum, plasma, body fluids.
  • Up to 5 x 10⁶ cultured cells.
Operation time
30 minutes
Binding capacity
up to 60 μg total DNA / column
Expected yield
4 ~ 8 μg / prep
Column applicability
centrifugation and vacuum
Minimum elution volume
50 μl

Kit components

HBBGK050HBBGK100HBBGK200HBBGK300
BG Buffer15 ml30 ml60 ml
GW Buffer (concentrate)22 ml44 ml88 ml
Wash Buffer (concentrate)10 ml20 ml40 ml
Elution Buffer15 ml30 pcs60 ml
Proteinase K11 mg11 mg x 211 mg x 4
BG Mini Column50 pcs100 pcs200 pcs
Collection Tube100 pcs200 pcs400 pcs
Elution Tube50 pcs100 pcs200 pcs
User Manual111

Handling notes

  • Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
  • Add 1.1 ml sterile ddH2O to Proteinase K tube to make a 10 mg/ml stock solution. Make sure that Proteinase K has been completely dissolved. Store the stock solution at 4 ℃ .
  • Add ethanol (96- 100 %) to GW Buffer and Wash Buffer when first open.
  • Prepare dry baths or water baths before the operation. One to 60℃ for step 2. Lysis.

Protocol

Brief procedure for Blood Genomic DNA Extraction Mini Kit
Brief procedure (click to enlarge)

Downloads

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