DNA/RNA Isolation Kits
Tissue Genomic DNA Extraction Mini Kit
Catalogue no.
Principlemini spin column (silica matrix)
Sample size
- Up to 25 mg animal tissue
- 1.2 cm mouse tail
- Up to 10⁷ cultured cells
Operation time30 ~ 60 minutes
Binding capacityup to 60 µg total DNA / column
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Offices in Delhi, Faridabad & Lucknow
Strict quality practices
Specifications
- Principle
- mini spin column (silica matrix)
- Sample size
- Up to 25 mg animal tissue
- 1.2 cm mouse tail
- Up to 10⁷ cultured cells
- Operation time
- 30 ~ 60 minutes
- Binding capacity
- up to 60 µg total DNA / column
- Expected yield
- 15 ~35 μg / prep
- Column applicability
- centrifugation and vacuum
- Minimum elution volume
- 50 μl
Kit components
| HBTGK050 | HBTGK100 | HBTGK200 | |
|---|---|---|---|
| TG1 Buffer | 15 ml | 30 ml | 60 ml |
| TG2 Buffer | 15 ml | 30 ml | 60 ml |
| Proteinase K (lyophilized) | 11mg | 11mg x 2 | 11mg x 4 |
| GW Buffer (concentrate) | 22 ml | 44 ml | 88 ml |
| Wash Buffer (concentrate) | 10 ml | 20 ml | 40 ml |
| Elution Buffer | 15 ml | 30 ml | 60 ml |
| TG Mini Column | 50 pcs | 100 pcs | 200 pcs |
| Collection Tube | 100 pcs | 200 pcs | 400 pcs |
| Elution Tube | 50 pcs | 100 pcs | 200 pcs |
| User Manual | 1 | 1 | 1 |
Handling notes
- Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
- Add 1.1 ml sterile ddH2O to Proteinase K tube to make a 10 mg/ml stock solution. Make sure that Proteinase K has been completely dissolved. Store the stock solution at 4 ℃ .
- Add ethanol (96- 100 %) to GW Buffer and Wash Buffer when first open.
- Prepare dry baths or water baths before the operation. One to 60℃ for step 2.1 and the other to 70℃ for step 2.2.
- Preheat the Elution Buffer to 70℃ for step 7.
- All centrifuge steps are done at full speed(~ 18,000 x g) in a microcentrifuge.
Protocol
Downloads
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