DNA/RNA Isolation Kits
Blood / Cultured Cell Genomic DNA Extraction Mini Kit
Catalogue no.
Principlemini spin column (silica matrix)
Sample size
- Up to 300 μl of Whole blood
- Up to 200 μl of frozen blood.
- Up to 200 μl of buffy coat
- Up to 1 x 10⁷ of Cultured animal cells
- Up to 1 x 10⁹ of Cultured bacterial cells
Operation time30 minutes
Binding capacityup to 60 μg total DNA / column
Toll free +91-1800-890-4669
Offices in Delhi, Faridabad & Lucknow
Strict quality practices
Specifications
- Principle
- mini spin column (silica matrix)
- Sample size
- Up to 300 μl of Whole blood
- Up to 200 μl of frozen blood.
- Up to 200 μl of buffy coat
- Up to 1 x 10⁷ of Cultured animal cells
- Up to 1 x 10⁹ of Cultured bacterial cells
- Operation time
- 30 minutes
- Binding capacity
- up to 60 μg total DNA / column
- Expected yield
- 4 ~ 8 μg / prep
- Column applicability
- centrifugation and vacuum
- Minimum elution volume
- 50 μl
Kit components
| HBBGR100 | HBBGR200 | |
|---|---|---|
| RBC Lysis Buffer | 135 ml | 270 ml |
| TG Buffer | 30 ml | 60 ml |
| BG Buffer | 40 ml | 80 ml |
| BW Buffer | 45 ml | 90 ml |
| Wash Buffer (concentrate) | 25 ml | 35 ml |
| Elution Buffer | 30 ml | 60 ml |
| BG Mini column | 100 pcs | 200 pcs |
| Collection Tube | 200 pcs | 400 pcs |
| User Manual | 1 | 1 |
Handling notes
- Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
- Add ethanol (96- 100 %) to GW Buffer and Wash Buffer when first open.
Protocol
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