Custom Oligo Synthesis

Overview

Helix provides a very flexible and abundant primer customization scheme. By reading this description, you can easily customize primer products to meet your application needs. And we can give you specialized guidance timely.

Synthesis scale and final yield (15–60 nt)

OPC purification

Purification with reverse-phase silica gel column which can effectively remove salts. The purity can meet the needs of most molecular biology experiments.

Advantages

• Fast
• High throughput
• Cost-effective

Applications: PCR amplification, gene synthesis, DNA sequencing, NGS capture probes, NGS Multiplex PCR oligos.

PAGE purification

Using denaturing polyacrylamide gel electrophoresis, oligo of different molecular weights migrate at different speeds during electrophoresis, allowing for separation. Finally, the target DNA is recovered from the gel.

Advantages

• Purity can be > 90%
• Effective for purification of long-stranded oligo

Applications: Mutation libraries, protein binding gel migration electrophoresis analysis, modified oligos such as phosphate and biotin.

HPLC purification

The purification of oligo with HPLC can achieve high purity and is mainly used for short chain and modified oligo.

Advantages

• Purity can reach > 90%
• Remove N-1 short fragment effectively
• Purification for modified oligo with hydrophobic groups

Applications: Point mutation, subcloning, diagnostic PCR, protein binding gel migration electrophoresis analysis, modified primers with hydrophobic groups.