DNA/RNA Isolation Kits
Tissue Total RNA Mini Kit
Catalogue no.
Principlemini spin column (silica matrix)
Sample sizePlease see Table. Sample amount and Yield.
Operation time30 ~ 60 minutes
Binding capacityup to 100 μg total RNA/ column
Toll free +91-1800-890-4669
Offices in Delhi, Faridabad & Lucknow
Strict quality practices
Specifications
- Principle
- mini spin column (silica matrix)
- Sample size
- Please see Table. Sample amount and Yield.
- Operation time
- 30 ~ 60 minutes
- Binding capacity
- up to 100 μg total RNA/ column
- Expected yield
- Please see Table. Sample amount and Yield.
- Column applicability
- centrifugation and vacuum
- Minimum elution volume
- 40 μl
Kit components
| HBTRK050 | HBTRK100 | |
|---|---|---|
| RL Buffer | 25 ml | 45 ml |
| Wash Buffer 1 | 30 ml | 60 ml |
| Wash Buffer 2 (concentrate) | 15 ml | 35 ml |
| RNase-free Water | 6 ml | 6 ml |
| Filter Column | 50 pcs | 100 pcs |
| RB Mini Column | 50 pcs | 100 pcs |
| Collection Tube | 100 pcs | 200 pcs |
| Elution Tube | 50 pcs | 100 pcs |
| User Manual | 1 | 1 |
Handling notes
- Make sure everything is RNase-free when handling RNA.
- Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
- Caution: ß-mercaptoethanol is hazardous to human health. perform the procedures involving RB Buffer or PRB Buffer in a chemical fume hood.
- Add required volume of RNase-free ethanol (96~100%) to Wash Buffer 2 when first use.
- Dilute RNase-free DNase 1 in reaction buffer (1M NaCl, 10 mM MnCl2, 20 mM Tris-HCl, pH 7.0 at 25ºC) to final conc. = 0.5 U/μl.
Protocol
Downloads
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