DNA/RNA Isolation Kits
High Yield Plasmid Extraction Mini Kit
Catalogue no.
Principlespin column (silica matrix)
Sample size1 ~ 5 ml
Size of plasmid or construct< 15 kb
Operation time< 25 minutes
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Offices in Delhi, Faridabad & Lucknow
Strict quality practices
Specifications
- Principle
- spin column (silica matrix)
- Sample size
- 1 ~ 5 ml
- Size of plasmid or construct
- < 15 kb
- Operation time
- < 25 minutes
- Typical Yield
- 25 ~ 40 µg
- Binding capacity
- 60 µg/ column
- Column applicability
- centrifugation and vacuum
Kit components
| HBHPD 100 | HBHPD 300 | |
|---|---|---|
| PD1 Buffer | 30 ml | 90 ml |
| PD2 Buffer | 30 ml | 90 ml |
| PD3 Buffer | 40 ml | 120 ml |
| PDW Buffer (concentrate) | 35 ml | 98 ml |
| Wash Buffer (concentrate) | 20 ml | 50 ml |
| Elution Buffer | 15 ml | 35 ml |
| PD Column | 100 pcs | 300 pcs |
| Collection Tube | 100 pcs | 300 pcs |
| RNase A (Lyophilized) | 3 mg | 9 mg |
| User Manual | 1 | 1 |
Handling notes
- Store RNase A at -20 °C upon receipt of kit.
- Add 0.5 ml of PD1 Buffer to a RNase A tube, Dissolve the RNase A by vortexing. Briefly spin the tube and transfer the total RNase A mixture back to the PD1 bottle, mix well by vortexing and store the PD1 buffer at 4 °C.
- If precipitates have formed in PD2 Buffer, warm the buffer in 37°C waterbath to dissolve precipitates.
- Preparation of PDW Buffer and Wash Buffer by adding 96 ~100% ethanol (not provided) for first use.
- Centrifugation steps are done by a microcentrifuge capable of the speed at 11,000 ~18,000 x g.
Protocol
Downloads
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