Gene Synthesis

Our extensive experience in gene synthesis technologies has allowed us to take on complex gene sequences. Unlike traditional molecular cloning technologies, Helix's gene synthesis service offers cost-effective solutions and quick turn-around time for your custom DNA synthesis, cloning, and/or recombinant expression project needs. We also offer a wide range of downstream services, including subcloning, mutagenesis, and plasmid preparations.

Service highlights

• Over 98% success rate
• Free codon optimization upon request
• Free subcloning into one of our in-stock vectors - 120+ vectors to choose from
• Fast turnaround time with 100% sequence accuracy guarantee
• Site directed mutagenesis service starting from Rs 19,500 + taxes
• One-stop for custom gene, peptide, protein, and antibody services

What you receive

• 4 µg of lyophilized plasmid containing the sequence verified gene insert
• QC data includes: project QA report, complete construct map, and sequence chromatogram

Turnaround time

Turnaround time (TAT) is an estimated time only; it does not reflect any production difficulties, if so encountered. Best price guarantee - we will match/beat a lower price from any reputable competitor.

Complimentary gene codon optimization

To achieve stable and enhanced heterologous protein expression levels, Helix offers complimentary codon optimization service to improve codon usage in one or multiple host cell systems. There are a variety of critical parameters taken into consideration, including but not limited to:

• Codon usage bias
• GC content
• CpG dinucleotide content
• mRNA secondary structure
• Cryptic splicing sites
• Premature PolyA sites
• Internal chi sites and ribosomal binding sites
• Negative CpG islands
• RNA instability motif (ARE)
• Repeat sequences (direct repeats, reverse repeats, and Dyad repeats)
• Restriction sites that may interfere with cloning

During the gene synthesis quotation request, simply request gene optimization and indicate the expression host species and we will provide a full optimized report for your review.

Cloning and expression vectors

Helix offers complimentary cloning into a standard cloning vector for each of your gene synthesis projects. We can also subclone your synthesized gene into one of our available expression vectors, for a small subcloning fee. Please note that all of our vectors are supplied free of charge, for laboratory research use only, but not to be used for any commercial purposes.

Standard workflow

1. Chemical synthesis of short oligonucleotides
2. Oligonucleotides annealing by ligation or polymerase reactions
3. Cloning of gene sequences into specific vectors
4. Screening of E. coli clones to identify putative candidates
5. Sequencing to verify correct plasmid sequence
6. Lyophilization of purified plasmid

Why choose Helix

Helix has been trusted among top scientists and researchers from around PAN India for quality products and custom services since 2013. To date, Helix has delivered 10,000+ custom made products to our PAN India customers.

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