DNA/RNA Isolation Kits
Plant Genomic DNA Extraction Mini Kit
Catalogue no.
Principlemini spin column (silica matrix)
Sample size
- Wet weight ≦ 100 mg
- Dry weight ≦ 20 mg
Operation time30 ~ 60 minutes
Binding capacityup to 60 μg total DNA / column
Toll free +91-1800-890-4669
Offices in Delhi, Faridabad & Lucknow
Strict quality practices
Specifications
- Principle
- mini spin column (silica matrix)
- Sample size
- Wet weight ≦ 100 mg
- Dry weight ≦ 20 mg
- Operation time
- 30 ~ 60 minutes
- Binding capacity
- up to 60 μg total DNA / column
- Expected yield
- 5 ~ 40 μg /prep
- Column applicability
- centrifugation and vacuum
- Minimum elution volume
- 50 μl
Kit components
| HBPGK050 | HBPGK100 | |
|---|---|---|
| PG1 Buffer | 25 ml | 55 ml |
| PG2 Buffer | 8 ml | 15 ml |
| PG3 Buffer (concentrate) | 15 ml | 30 ml |
| GW Buffer (concentrate) | 13 ml | 26 ml |
| Wash Buffer (concentrate) | 15 ml | 30 ml |
| Elution Buffer | 15 ml | 30 ml |
| RNase A (lyophilized ) | 22 mg | 43 mg |
| filter Column | 50 pcs | 100 pcs |
| PG Column | 50 pcs | 100 pcs |
| Collection Tube | 100 pcs | 200 pcs |
| User Manual | 1 | 1 |
Handling notes
- Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
- Check PG1 Buffer before use, Warm PG1 Buffer at 60℃ for 5 min if any precipitate formed.
- Preheat dry baths or water baths to 65℃ before the operation.
- Add required ethanol (96-100%) to PG3 Buffer, GW and Wash before use.
- Store RNase A at -20℃ upon receipt of kit. Add sterile ddH2O to RNase A tube to make a 50 mg/ml stock solution. Vortex and make sure that RNase A has been completely dissolved. Store the stock solution at -20℃ .
- All centrifuge steps are done at full speed(~ 18,000 x g) in a microcentrifuge.
Protocol
Downloads
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